Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.
Identifieur interne : 000E57 ( Main/Exploration ); précédent : 000E56; suivant : 000E58Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.
Auteurs : María Josefina Iribarren [Argentine] ; Cecilia Pascuan [Argentine] ; Gabriela Soto [Argentine] ; Nicolás Daniel Ayub [Argentine]Source :
- FEMS microbiology letters [ 1574-6968 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Données de séquences moléculaires (MeSH), Facteurs de virulence (génétique), Ilots génomiques (MeSH), Légumes (microbiologie), Microbiologie de l'environnement (MeSH), Phytophthora (génétique), Phytophthora (isolement et purification), Plasmides (MeSH), Réaction de polymérisation en chaîne (MeSH), Séquences répétées dispersées (MeSH), Évolution moléculaire (MeSH).
- MESH :
- génétique : Facteurs de virulence, Phytophthora.
- isolement et purification : Phytophthora.
- microbiologie : Légumes.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Ilots génomiques, Microbiologie de l'environnement, Plasmides, Réaction de polymérisation en chaîne, Séquences répétées dispersées, Évolution moléculaire.
English descriptors
- KwdEn :
- Environmental Microbiology (MeSH), Evolution, Molecular (MeSH), Genomic Islands (MeSH), Interspersed Repetitive Sequences (MeSH), Molecular Sequence Data (MeSH), Phytophthora (genetics), Phytophthora (isolation & purification), Plasmids (MeSH), Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH), Vegetables (microbiology), Virulence Factors (genetics).
- MESH :
- chemical , genetics : Virulence Factors.
- genetics : Phytophthora.
- isolation & purification : Phytophthora.
- microbiology : Vegetables.
- Environmental Microbiology, Evolution, Molecular, Genomic Islands, Interspersed Repetitive Sequences, Molecular Sequence Data, Plasmids, Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora.
DOI: 10.1093/femsle/fnv189
PubMed: 26443834
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.</title><author><name sortKey="Iribarren, Maria Josefina" sort="Iribarren, Maria Josefina" uniqKey="Iribarren M" first="María Josefina" last="Iribarren">María Josefina Iribarren</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Universidad Nacional de Luján, Buenos Aires, CP1428, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Universidad Nacional de Luján, Buenos Aires, CP1428</wicri:regionArea><wicri:noRegion>CP1428</wicri:noRegion></affiliation></author><author><name sortKey="Pascuan, Cecilia" sort="Pascuan, Cecilia" uniqKey="Pascuan C" first="Cecilia" last="Pascuan">Cecilia Pascuan</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author><author><name sortKey="Soto, Gabriela" sort="Soto, Gabriela" uniqKey="Soto G" first="Gabriela" last="Soto">Gabriela Soto</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author><author><name sortKey="Ayub, Nicolas Daniel" sort="Ayub, Nicolas Daniel" uniqKey="Ayub N" first="Nicolás Daniel" last="Ayub">Nicolás Daniel Ayub</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina ayub.nicolas@inta.gob.ar.</nlm:affiliation><country wicri:rule="url">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26443834</idno><idno type="pmid">26443834</idno><idno type="doi">10.1093/femsle/fnv189</idno><idno type="wicri:Area/Main/Corpus">000D49</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000D49</idno><idno type="wicri:Area/Main/Curation">000D49</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000D49</idno><idno type="wicri:Area/Main/Exploration">000D49</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.</title><author><name sortKey="Iribarren, Maria Josefina" sort="Iribarren, Maria Josefina" uniqKey="Iribarren M" first="María Josefina" last="Iribarren">María Josefina Iribarren</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Universidad Nacional de Luján, Buenos Aires, CP1428, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Universidad Nacional de Luján, Buenos Aires, CP1428</wicri:regionArea><wicri:noRegion>CP1428</wicri:noRegion></affiliation></author><author><name sortKey="Pascuan, Cecilia" sort="Pascuan, Cecilia" uniqKey="Pascuan C" first="Cecilia" last="Pascuan">Cecilia Pascuan</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author><author><name sortKey="Soto, Gabriela" sort="Soto, Gabriela" uniqKey="Soto G" first="Gabriela" last="Soto">Gabriela Soto</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author><author><name sortKey="Ayub, Nicolas Daniel" sort="Ayub, Nicolas Daniel" uniqKey="Ayub N" first="Nicolás Daniel" last="Ayub">Nicolás Daniel Ayub</name><affiliation wicri:level="1"><nlm:affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina ayub.nicolas@inta.gob.ar.</nlm:affiliation><country wicri:rule="url">Argentine</country><wicri:regionArea>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712</wicri:regionArea><wicri:noRegion>CP1712</wicri:noRegion></affiliation></author></analytic><series><title level="j">FEMS microbiology letters</title><idno type="eISSN">1574-6968</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Environmental Microbiology (MeSH)</term><term>Evolution, Molecular (MeSH)</term><term>Genomic Islands (MeSH)</term><term>Interspersed Repetitive Sequences (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phytophthora (genetics)</term><term>Phytophthora (isolation & purification)</term><term>Plasmids (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Vegetables (microbiology)</term><term>Virulence Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteurs de virulence (génétique)</term><term>Ilots génomiques (MeSH)</term><term>Légumes (microbiologie)</term><term>Microbiologie de l'environnement (MeSH)</term><term>Phytophthora (génétique)</term><term>Phytophthora (isolement et purification)</term><term>Plasmides (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquences répétées dispersées (MeSH)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de virulence</term><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Légumes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Vegetables</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Environmental Microbiology</term><term>Evolution, Molecular</term><term>Genomic Islands</term><term>Interspersed Repetitive Sequences</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Ilots génomiques</term><term>Microbiologie de l'environnement</term><term>Plasmides</term><term>Réaction de polymérisation en chaîne</term><term>Séquences répétées dispersées</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26443834</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>25</Day></DateCompleted><DateRevised><Year>2015</Year><Month>10</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1574-6968</ISSN><JournalIssue CitedMedium="Internet"><Volume>362</Volume><Issue>22</Issue><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>FEMS microbiology letters</Title><ISOAbbreviation>FEMS Microbiol Lett</ISOAbbreviation></Journal><ArticleTitle>Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1093/femsle/fnv189</ELocationID><ELocationID EIdType="pii" ValidYN="Y">fnv189</ELocationID><Abstract><AbstractText>Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora. </AbstractText><CopyrightInformation>© FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Iribarren</LastName><ForeName>María Josefina</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Universidad Nacional de Luján, Buenos Aires, CP1428, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pascuan</LastName><ForeName>Cecilia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soto</LastName><ForeName>Gabriela</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ayub</LastName><ForeName>Nicolás Daniel</ForeName><Initials>ND</Initials><AffiliationInfo><Affiliation>CONICET, Cuidad Autónoma de Buenos Aires, CP1661, Argentina Instituto de Genética Ewald A. Favret, Buenos Aires, CP1712, Argentina ayub.nicolas@inta.gob.ar.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KF746518</AccessionNumber><AccessionNumber>KF746519</AccessionNumber><AccessionNumber>KF746520</AccessionNumber><AccessionNumber>KF746521</AccessionNumber><AccessionNumber>KF746522</AccessionNumber><AccessionNumber>KF746523</AccessionNumber><AccessionNumber>KF746524</AccessionNumber><AccessionNumber>KF746525</AccessionNumber><AccessionNumber>KF746526</AccessionNumber><AccessionNumber>KF746527</AccessionNumber><AccessionNumber>KF746528</AccessionNumber><AccessionNumber>KF746529</AccessionNumber><AccessionNumber>KF746530</AccessionNumber><AccessionNumber>KF746531</AccessionNumber><AccessionNumber>KF746532</AccessionNumber><AccessionNumber>KF746533</AccessionNumber><AccessionNumber>KF746534</AccessionNumber><AccessionNumber>KF746535</AccessionNumber><AccessionNumber>KF746536</AccessionNumber><AccessionNumber>KF746537</AccessionNumber><AccessionNumber>KF746538</AccessionNumber><AccessionNumber>KF746539</AccessionNumber><AccessionNumber>KF746540</AccessionNumber><AccessionNumber>KF746541</AccessionNumber><AccessionNumber>KF746542</AccessionNumber><AccessionNumber>KF746543</AccessionNumber><AccessionNumber>KF746544</AccessionNumber><AccessionNumber>KF746545</AccessionNumber><AccessionNumber>KF746546</AccessionNumber><AccessionNumber>KF746547</AccessionNumber><AccessionNumber>KF746548</AccessionNumber><AccessionNumber>KF746549</AccessionNumber><AccessionNumber>KF746550</AccessionNumber><AccessionNumber>KF746551</AccessionNumber><AccessionNumber>KF746552</AccessionNumber><AccessionNumber>KF746553</AccessionNumber><AccessionNumber>KF746554</AccessionNumber><AccessionNumber>KF746555</AccessionNumber><AccessionNumber>KF746556</AccessionNumber><AccessionNumber>KF746557</AccessionNumber><AccessionNumber>KF746558</AccessionNumber><AccessionNumber>KF746559</AccessionNumber><AccessionNumber>KF746560</AccessionNumber><AccessionNumber>KF746561</AccessionNumber><AccessionNumber>KF746562</AccessionNumber><AccessionNumber>KF746563</AccessionNumber><AccessionNumber>KF746564</AccessionNumber><AccessionNumber>KF746565</AccessionNumber><AccessionNumber>KF746566</AccessionNumber><AccessionNumber>KF746567</AccessionNumber><AccessionNumber>KF746568</AccessionNumber><AccessionNumber>KF746569</AccessionNumber><AccessionNumber>KF746570</AccessionNumber><AccessionNumber>KF746571</AccessionNumber><AccessionNumber>KF746572</AccessionNumber><AccessionNumber>KF746573</AccessionNumber><AccessionNumber>KF746574</AccessionNumber><AccessionNumber>KF746575</AccessionNumber><AccessionNumber>KF746576</AccessionNumber><AccessionNumber>KF746577</AccessionNumber><AccessionNumber>KF746578</AccessionNumber><AccessionNumber>KF746579</AccessionNumber><AccessionNumber>KF746580</AccessionNumber><AccessionNumber>KF746581</AccessionNumber><AccessionNumber>KF746582</AccessionNumber><AccessionNumber>KF746583</AccessionNumber><AccessionNumber>KF746584</AccessionNumber><AccessionNumber>KF746585</AccessionNumber><AccessionNumber>KF746586</AccessionNumber><AccessionNumber>KT441013</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Lett</MedlineTA><NlmUniqueID>7705721</NlmUniqueID><ISSNLinking>0378-1097</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004783" MajorTopicYN="Y">Environmental Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044404" MajorTopicYN="Y">Genomic Islands</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020071" MajorTopicYN="N">Interspersed Repetitive Sequences</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014675" MajorTopicYN="N">Vegetables</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AVR3a1</Keyword><Keyword MajorTopicYN="N">Phytophthora capsici</Keyword><Keyword MajorTopicYN="N">RXLR effector</Keyword><Keyword MajorTopicYN="N">genomic island</Keyword><Keyword MajorTopicYN="N">integrase</Keyword><Keyword MajorTopicYN="N">vegetable crops</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>10</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26443834</ArticleId><ArticleId IdType="pii">fnv189</ArticleId><ArticleId IdType="doi">10.1093/femsle/fnv189</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><country name="Argentine"><noRegion><name sortKey="Iribarren, Maria Josefina" sort="Iribarren, Maria Josefina" uniqKey="Iribarren M" first="María Josefina" last="Iribarren">María Josefina Iribarren</name></noRegion><name sortKey="Ayub, Nicolas Daniel" sort="Ayub, Nicolas Daniel" uniqKey="Ayub N" first="Nicolás Daniel" last="Ayub">Nicolás Daniel Ayub</name><name sortKey="Pascuan, Cecilia" sort="Pascuan, Cecilia" uniqKey="Pascuan C" first="Cecilia" last="Pascuan">Cecilia Pascuan</name><name sortKey="Soto, Gabriela" sort="Soto, Gabriela" uniqKey="Soto G" first="Gabriela" last="Soto">Gabriela Soto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E57 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E57 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26443834
|texte= Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26443834" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |